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Curr Opin Nephrol Hypertens 2020;29:128–135

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The cells were cultured in Eagle’s minimum essential medium (ThermoFisher Scientific) or DMEM F-12 (ThermoFisher Scientific) respectively, supplemented with 10% Fetal Bovine Serum (Gibco) and 1% penicillin–streptomycin (ThermoFisher Scientific), in a humidified incubator maintaining 5% CO 2 at 37 °C